Synembryn-B (UniProt Q80XE1; also known as Protein Ric-8B, Resistance to inhibitors of cholinesterase 8B) is encoded by the Ric8b (also known as BC051080) gene (Gene ID 237422) in murine species. Ric-8A and Ric-8B stimulate nucleotide exchange of all Galpha subunit classes by stabilizing the Galpha nucleotide-free transition state. Ric-8A acts upon Galphai/q/13, while Ric-8B targets Galphas/olf. In addition to their function as guanine nucleotide exchange factors (GEFs), Ric proteins also act as molecular chaperones that directly participate in the posttranslational folding of nascent Galpha subunits to promote G-protein biosynthesis and membrane association. Ric-8 knockdown reduces Galpha steady-state abundances and overexpression of Ric-8 proteins enhances cellular Galpha levels. In Ric-8A- or Ric-8B-delicient ES cells, newly produced Galpha subunits are defective in the initial association with an internal membrane, resulting in a >=10-fold faster turned over rate than in WT cells.
Synonyms: Synembryn-B, Protein Ric-8B, Ric-8B, Ric-8B/Synembryn-B
Application: Western Blotting Analysis: A representative lot detected Ric-8B protein in murine embryonic stem (mES) cells (mESCs) derived from wild-type (WT) and Ric-8A-/- mice, but not mESCs from Ric-8B-/- mice (Gabay, M., et al. (2011). Sci Signal.4(200):ra79). Western Blotting Analysis: A representative lot detected the endogenous Ric-8B protein in rabbit reticulocyte lysates (RRL) as well as recombinant murine Ric-8B protein (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).Immunoprecipitation Analysis: A representative lot immunoprecipitated the endogenous Ric-8B protein from rabbit reticulocyte lysates/RRL (Chan, P., et al. (2013). Proc Natl Acad Sci U S A. 110(10):3794-3799).
Other Notes: Concentration: Please refer to lot specific datasheet.