This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be<10 pg micro;l by the lal method. the recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kda. by western blot from a reducing gel expect a band approximately 15.0 kda in size corresponding to rat il17-a protein in the appropriate cell lysate or extract. specific conditions for reactivity should be optimized by the end user.<>