Anti-Rat IgM (mu-chain specific)-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder

Synonyms: HRP

Storage: 2-8C

UNSPSC Code: 12352203

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General description: Immunoglobulin M (IgM) exists as a pentamer in the serum and has a high molecular weight. Monomeric IgM has a molecular weight of 190kDa. The pentameric IgM consists of ten ++-heavy chains, ten light chains and a single J-chain which are linked by disulphide bonds. The ++-chain consists of five domains- VH, C++1, C++2, C++3 and C++4. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as Fragment antigen binding (Fab) fragments. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The heavy chains of IgM contain an extra C domain that replaces the hinge region present between Fab and Fc. IgM is the first antibody to be produced during an immune response.

Immunogen: Rat IgM mu heavy chain

Physical form: Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Physical properties: Antibody format: IgG

Reconstitution: Reconstitute with 1.0 mL deionized water (or equivalent).

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes.