PNKP (Polynucleotide kinase 3'-phosphatase) is a 57.1kDa, DNA repair protein. It consists of ATP binding, kinase domain at the C-terminal end, a potential 3'-phosphatase central domain and a fork-head associated (FHA) domain at the N-terminus end.
Synonyms: Anti-Bifunctional polynucleotide phosphatase/kinase; Anti-DNA 5'-kinase/3'-phosphatase; Anti-Polynucleotide kinase- 3'-phosphatase
Storage: -20C
Application: All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
Biochem Physiol Actions: PNKP (Polynucleotide kinase 3'-phosphatase) is majorly involved in the DNA repair caused by ionizing radiation or oxidative damage. It possesses two enzymatic activities, a DNA 5' kinase activity and a 3' phosphatase activity. In DNA repair, it functions in the two processes, single-strand break repair and non-homologous telomeric end joining for double-strand break repair. Mutation in the PNKP gene mainly alters the DNA repair process and also causes microcephaly and seizures.
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