Anti-Phosphotyrosine antibody produced in rabbit, 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

MDL No.: MFCD00164715

Storage: -20C

UNSPSC Code: 12352203

General description: Protein phosphorylation is a post-translational modification that regulates signal transduction. Phosphorylation of tyrosine residues in proteins has been associated with oncogenesis, cell growth and survival . Anti-Phosphotyrosine recognizes tyrosine phosphorylated proteins. The antibody detects phospho-L-tyrosine, but does not recognize phospho-L-serine, phospho-L-threonine and L-tyrosine by immunoblotting.

Application: Anti-Phosphotyrosine antibody is suitable for use in indirect immunofluorescence (3-6 μg/mL using A431 stiμLated by human EGF) and immunoblot (using U2OS Dr1 cells ). The antibody may also be used for immunoprecipitation (1:40 using rat lumbar and thoracic lysates) and immunocytochemistry.

Immunogen: phospho-L-tyrosine conjugated to KLH

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, and 1% BSA, containing 15 mM sodium azide.