Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the PT-66 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with phosphotyrosine-BSA conjugate.
Synonyms: Monoclonal Anti-Phosphotyrosine; Phospho-Tyr; Phospho-tyrosine; p-Tyr
Storage: -20C
Application: Monoclonal Anti-Phosphotyrosine has been used in
•immunoblotting,
•immunofluorescence,
•immunohistochemistry
•immunocytochemistry
•flow cytometry
•immunoprecipitation
•enzyme linked immunosorbent assay (ELISA)
•radio immunoassay (RIA)
•immunoaffinity isolation
Biochem Physiol Actions: Protein phosphorylation is a basic signaling mechanism that modifies protein function in eukaryotic cells. Serine, threonine, and tyrosine are the major phosphorylated amino acids in proteins. Tyrosine phosphorylation is a rare post-translational event in normal tissues, accounting for only 0.03% of phosphorylated amino acids. However, this phosphorylation increases several folds by various activation signals and the process is mediated by protein tyrosine kinases. Protein-tyrosine kinases (PTKs) are enzymes that catalyze the transfer of ?-phosphate of ATP to tyrosine residues of protein substrates. The PTKs are responsible for many biological processes like cell cycle, proliferation, oncogenesis, and development.