ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.
Synonyms: H3/g; H3t; Histone H3/t
Storage: 2-8C
Application: Flow Cytometry Analysis: 0.01 mug from a representative lot detected phospho-Histone H3 (Ser10) in one million Jurkat cells.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H3 (Ser10) in HeLa, A431, HUVEC, and NIH 3T3 cells.Dot Blot Analysis: Representative lot was tested on AbSurance Histone Peptide Microarray.Peptide Inhibition Assay Analysis: 1:100 dilution from a representative lot did not detect the target band in acid extract from HeLa cells treated with colemid (50 ng/ml) when preblocked with the S10 phosphopeptide, but detected the band when the corresponding non-phosphopeptide was used.Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany