Developmentally-regulated brain protein, also known as Drebrin, and encoded by the gene name DBN1, is a developmentally-regulated neuron-specific protein, expressed during outgrowth and maturation of dendrites, and localized within dendrites of the adult brain. Drebrin is required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. Recent studies have shown that Drebrins may play a role in cell migration, extension of neuronal processes and plasticity of dendrites. Drebrins are classified into two forms of the embryonic type (E1 and E2) and one form of the adult type (A). The time course of their appearance are different from each other. Their structures are closely related. Adult rat brain expresses only drebrin A while drebrin E1 or E2 is observed in immature animals. Drebrin E+A- signals have been observed in the cell bodies of migrating neuroblasts in the core region; however, only fibrous and punctate drebrin E+A- signals have been observed in postmigratory neuroblasts at the outer layers. Studies have demonstrated that the disappearance of Drebrin E+A- signals from the cell body coincides with the cessation of neuronal migration. The disappearance of Drebrin E from the cell body may be a molecular switch for the cessation of migration in newly generated neuroblast. Drebrin is expressed mainly in brain neurons.
Synonyms: Drebrin, Developmentally-regulated brain protein
Application: Western Blotting Analysis: 0.5 µg/mL from a representative lot detected phospho Drebrin (Ser142) in 10 µg of rat neonatal brain tissue lysate.Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected phospho Drebrin (Ser142) in HeLa and NIH/3T3 cells.Peptide Inhibition Analysis: 0.5 µg/mL from a representative lot detected phospho Drebrin (Ser142) in 10 µg of rat neonatal brain tissue lysate.Immunocytochemistry Analysis: A representative lot detected phospho Drebrin (Ser142) in rat embryonic cortical culture (Prof. Phillip R. Gordon-Weeks, King's College London).
Other Notes: Concentration: Please refer to lot specific datasheet.