Anti-PD-1 Antibody has been tested for use in ELISA, Western Blotting, Immunofluorescence, and Immunohistochemistry. Expect a band at approximately 32 kDa in Western Blots using positive control THP-1 whole cell lysate, Human brain tissue for IHC, or other specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user.