This antibody reacts with human and mouse PARC by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). To date co-immunoprecipitation using 35S-IP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control. Researchers should determine optimal titers for other applications.