This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA, the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse NF2 (Merlin). Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.