Myosin-2 (UniProt Q9BE41; also known as MyHC-2a, MyHC-IIa, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa, Myosin heavy chain, skeletal muscle, adult 2) is encoded by the MYHSA gene (Gene ID 788772) in bovine species. Myosin heavy chain (MyHC) is a major structural component of the striated muscle contractile apparatus and is essential for body movement and cardiac contractility. MyHC are encoded by a highly conserved multigene family, of which eight isoforms have been identified in mammals, each encoded by a separate gene that displays distinct temporal-spatial regulation. MyHC-2a is expressed in the fast-type 2A muscle fibers. Recessive MyHC-2a myopathy caused by missense mutations results in mild muscle weakness. Autosomal dominant mutation (E706K) is reported to cause defective muscle function and compromises the structural integrity of all muscle cells. Ref.: Tajsharghi, H et al. (2014). Eur. J. Hum. Genet. 22: 801-808.
Synonyms: Myosin-2, MyHC-2a, MyHC-IIa, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa, Myosin heavy chain, skeletal muscle, adult 2
Application: Western Blotting Analysis: 0.5 microg/mL from a representative lot detected Myosin-2 (MYH2) in 10 microg of human gastrocnemious muscle and showed minimal reactivity with soleus embryonic muscle tissue lysates (Courtesy of Alberto Rossi, Ph.D., University of Colorado, U.S.A.).Immunofluorescence Analysis: A representative lot immunostained type 2A fibers in mouse hindlimb muscle cryosections encompassing soleus and surrounding tissues (Kurapati, R., et al. (2012). Hum. Mol. Genet. 21(8):1706-1724).Immunofluorescence Analysis: A representative lot immunostained type 2A fibers in rat soleus muscle cryosections following Bupivacaine-induced muscle regeneration. In tetrodotoxin/TTX-paralyzed-regenerated muscles type 2A MHC was not expressed (Midrio, M., et al. (2002). Basic Appl. Myol. 12(2): 77-80).Immunohistochemistry Analysis: A representative lot detected type IIA myosin heavy chain (MyHC) in human masseter (jaw) muscle cryosections (Horton, M.J., et al. (2001). Arch. Oral Biol. 46(11):1039-1050).Immunohistochemistry Analysis: Representative lots immunostained type 2A, but not type 1, 2B, or 2X, fibers in soleus (rat) and tibialis (mouse, rat, and Mgray short-tailed opossum/Monodelphis domestica) anterior muscle cryosections. Clone SC-71 failed to stain guinea pig tibialis sections (Sciote, J.J., and Rowlerson, A. (1998). Anat. Rec. 251(4):548-562; Gorza, L. (1990). J. Histochem Cytochem. 38(2):257-265; Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205).Western Blotting Analysis: A representative lot detected type IIA myosin heavy chain (MyHC) in human masseter (jaw) single muscle fibres extract (Horton, M.J., et al. (2001). Arch. Oral Biol. 46(11):1039-1050).Western Blotting Analysis: A representative lot detected myosin heavy chain (MHC) in myosin preparations from rat diaphragm, as well as the light meromyosin and rod, but not heavy meromyosin or S-1, fragments of MHC (Schiaffino, S., et al. (1989). J. Muscle Res. Cell Motil. 10(3):197-205).
Other Notes: Concentration: Please refer to lot specific datasheet.