Anti-mTOR antibody produced in rabbit, 1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-FKBP-12 rapamycin associated protein; Anti-FRAP; Anti-RAFT; Anti-SEP

MDL No.: MFCD02263996

Storage: -20C

UNSPSC Code: 12352203

General description: mTOR is an evolutionary conserved serine/threonine kinase that belongs to the phosphoinositol kinase-related kinase (PIKK) family. This kinase regulates cell growth by initiation of translation in response to nutrients such as amino acids (primarily leucine), growth factors, mitogens and insulin. Translational initiation is mediated by mTOR through S6K1 activation and 4E-BP1 inhibition. mTOR may also modulate several cellular functions such as actin organization, secretion, membrane trafficking, protein degradation, protein kinase C signaling, tRNA synthesis and ribosome biogenesis. Anti-mTOR antibody is specific for human, mouse and rat mTOR (289 kDa).

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Application: Anti-mTOR antibody is suitable for use in Western blot (1:1,000 using myotube cell lysates of rainbow trout cultures). The antibody can also be used in indirect immunofluorescence, immunoprecipitation, and microarray.

Immunogen: synthetic peptide corresponding to amino acid residues 2433-2450 of human mTOR.

Immunogen: The corresponding sequence is identical in mouse and differs by one amino acid in rat.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Specificity: Anti-mTOR (FRAP) is produced using a peptide corresponding to amino acids 2433-2450 of human mTOR. The corresponding sequence is identical in mouse and differs by one amino acid in rat. The antibody specifically recognizes human, mouse and rat mTOR (289 kDa) and is suitable for immunoblotting, immunoprecipitation and immunofluorescence.