Anti-Mouse IgG (whole molecule)-Peroxidase antibody produced in goat, IgG fraction of antiserum, buffered aqueous solution

Synonyms: Anti Mouse Hrp Sigma

MDL No.: MFCD00162644

Storage: -20C

UNSPSC Code: 12352203

General description: IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid. These antibodies are capable of fighting bacterial and viral infection. They also regulate several functions like placental transport, cell surface-receptor binding and complement activation. Anti-mouse IgG (whole molecule)-peroxidase antibody can be used in ELISA (diluted 1: 200 in PBST) . It can also be used in western blotting. Goat anti-mouse IgG-peroxidase antibody reacts specifically with mouse IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM myeloma proteins.

Application: Western blot analysis using HRP conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:3000 in BSA/PBS was performed to detect IgG antibodies against outer-membrane vexicle proteins.

Application: Anti-mouse IgG (whole molecule)-peroxidase antibody (diluted 1: 300) can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.

Immunogen: Purified mouse IgG.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.01% thimerosal.

Preparation Note: Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).