MDL No.: MFCD00162638
Storage: -20C
UNSPSC Code: 12352203
General description: Immunoglobulin G (IgG) is a member of the immunoglobulin family and contains gamma (γ) heavy chain in the constant (C) region. Monomeric structure of IgG contains two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively. The primary structure of this antibody also contains disulfide bonds implicated in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3 and IgG4 with different heavy chains, named γ1, γ2, γ3 and γ4, respectively.
Application: Anti-Mouse IgG (whole molecule) antibody produced in rabbit was used as secondary antibody in immunoblotting, immunoprecipitation and immunohistochemistry.
Biochem/physiol Actions: IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunogen: Anti-Mouse IgG is produced in rabbit using purified mouse IgG as the immunogen. Whole antiserum is purified to provide an IgG fraction of antiserum.
Physical form: Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as preservative
Specificity: The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) versus normal mouse serum and mouse IgG. By Ouchterlony Double Diffusion (ODD) the product reacts with mouse IgG, mouse IgA and IgM.
Preparation Note: Storage and Stability: For continuous use, store at 2-8 C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Other Notes: Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the product followed by diffusion versus the anti-rabbit IgG and the anti-rabbit whole serum results in single arcs of precipitation in the gamma region. Each milliliter of antiserum contains 1.8-2.5 mg of specific antibody. Normal mouse serum is used to determine the antibody concentration by a quantitative precipitation assay.Protein concentration: determined by OD at 280 nm.
RIDADR: NONH for all modes of transport
WGK Germany: 3