Anti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
Application: Western Blotting Analysis: 0.4 µg/mL of a representative lot detected mono-ADP-ribosylated proteins. Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Other Notes: Concentration: Please refer to lot specific datasheet.