MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).
Synonyms: CD146 antigen, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, melanoma adhesion molecule, melanoma cell adhesion molecule
Storage: 2-8C
Application: Immunocytochemistry: 1-10 mug/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells. Immunohistochemistry: 1-10 µg/mL. 4% PFA for 30min RT or<2hrs at 4c. block w 1%bsa .2% tween20 bs for 30min. works well in frozen tissue; fixed or unfixed. immunoprecipitation: 1-10 mug l of a previous lot worked in immunoprecipitation.elisa: 1-10 mug l of a previous lot worked in elisa.facs analysis: 1-10 mug l of a previous lot worked in facs.optimal working dilutions must be determined by end user.<>
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Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.