MANF (mesencephalic astrocyte-derived neurotrophic factor) forms a family of neurotrophic factors (NTFs) along with cerebral dopamine neurotrophic factor (CDNF). It is characterized by eight conserved cysteine residues, a saposin-like domain in the N-terminal and a putative unfolded carboxy-terminal domain. This protein has a 21 amino acids long signal peptide, which is cleaved off to yield a mature protein of 158 amino acids. MANF is a secreted protein, but some of it remains in endoplasmic reticulum (ER). It was initially isolated from cultured embryonic dopaminergic neurons, and in brain, is predominantly expressed in cerebral cortex, hippocampus and cerebellar Purkinje cells. This gene is localized to human chromosome 3, spans 4.3kb, and contains 4 exons. The encoded protein has a molecular weight of 20kDa.
Synonyms: Anti-ARMET; Anti-Arginine-rich protein; Anti-MANF; Anti-Mesencephalic astrocyte-derived neurotrophic factor; Anti-Protein ARMET precursor
Storage: -20C
Application: All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
Biochem Physiol Actions: MANF (mesencephalic astrocyte-derived neurotrophic factor) plays a protective role towards midbrain dopaminergic neurons. It is an endoplasmic reticulum (ER) stress related protein. Its levels are increased post brain injury, ischemia and epileptic insults. It might confer neuro-protection against brain ischemia, by preventing necrosis or apoptosis of cerebral cortex cells. It is over-expressed during unfolded protein response (UPR), and prevents ER stress-induced apoptosis, as well as cell proliferation.
Legal Information: Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC