Anti-LC3B Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Positive control used U251-MG Cells in Flow and Human lymph node in IHC. Testing only recommended in WB when using recombinant or overexpressing systems. Expect a band at ~14.6 kDa in western blot using appropriate lysates or tissues.