Anti-L-Asparaginase has been assayed against 1.0 ug of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,200 to 1:12,800 of the reconstitution concentration is suggested for this product.