Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
Synonyms: CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Storage: 2-8C
Application: Immunoprecipitation: A previous lot of this anitbody was used to immunoprecipitate VLA (beta1) integrins from lysate of 106 cell equivalent: 2 mug.Flow cytometry: A 10 mug/mL concentration of a previous lot was used in FC.Immunohistochemistry in frozen tissue sections: 20 mug/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (beta1), binding of MAB1997 to diverse cell types is commonly observed.Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (beta1) integrin signaling properties by MAB1997 has not been characterized. Western blot: 10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples. Optimal working dilutions must be determined by end user.
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Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.