Storage: -10 to -25C
Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected IL-18 in HeLa cell lysate.Tested ApplicationsImmunoprecipitation Analysis: A representative lot immunoprecipitated IL-18 in Immunoprecipitation applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A representative lot detected IL-18 in Immunocytochemistry applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Western Blotting Analysis: A representative lot detected IL-18 in Western Blotting applications (Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82).Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected IL-18 in HeLa cells.Inhibition Assay: A representative lot inhibited IL-18 involved interferon release from KG-1 cells.(Nariai, Y., et. al. (2019). Arch Biochem Biophys. 663:71-82). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.