This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be<10 pg micro;l by the lal method. by western blot a band approximately 15 kda in size corresponding to native human il-3 protein is expected in the appropriate cell lysate or extract. specific conditions for reactivity should be optimized by the end user.<>