IL-10 antiserum has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may be useful in radioimmunoassay, neutralizations, immunoprecipitation, immunohistochemistry. Reactivity in other immunoassays is unknown. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-10 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended.