Anti-Human IgG (Fc specific)-Peroxidase antibody produced in goat, affinity isolated antibody

Synonyms: Anti Human IgG Antibody - Anti-Human IgG (Fc specific)-Peroxidase antibody produced in goat; Anti Human Igg; Anti Human Igg Hrp; Anti-Human Igg

MDL No.: MFCD00162439

Storage: -20C

UNSPSC Code: 12352203

General description: Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.The antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and is purified to remove unconjugated material.Specificity and cross-reactivity of the Anti-Human IgG (Fc specific)-Peroxidase is determined by ELISA. The conjugate is specific for human IgG (Fc fragment) when tested against human IgA, IgG (Fab and Fc fragments), IgM, Bence Jones kappa, and lambda myeloma proteins. Furthermore, the conjugate shows no reactivity with mouse or rat IgG and yields reduced background with mouse or rat samples.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Enzyme-linked immunosorbent assay (12 papers)Western Blotting (3 papers)

Immunogen: Fc segment of human IgG

Physical form: Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.01% thimerosal

Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).