Anti-Human IgG F(ab')2 antibody has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product. This product is also suitable for Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.