Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat, affinity isolated antibody

Synonyms: Anti Human Fab Antibody; Anti Human Fab Antibody - Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat

MDL No.: MFCD00162434

Storage: -20C

UNSPSC Code: 12352203

General description: Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,.Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.Specificity of the Anti-Human IgG (Fab specific)-Peroxidase is determined by ELISA. Before conjugation, the antibody is found to react with human serum, human IgA, IgG (whole molecule and Fab fragment), IgM, Bence Jones kappa and lambda myeloma proteins using immunoelectrophoresis (IEP). No reactivity is observed with the Fc fragment of human IgG. The antibody does not react with mouse and rat IgG and yields reduced background with mouse or rat samples.

Application: Goat anti-human IgG (Fab specific)-peroxidase antibody can be used for direct ELISA (1:40,000), dot blot and immunohistochemistry (formalin-fixed, paraffin-embedded sections, 1:200) applications.

Application: Peroxidase-conjugated goat anti-human IgG (Fab specific) antibody was used for western blot and ELISA analysis of yeast cultures genetically modified to produced recombinant Fab fragments. Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene- diamine substrate solution (Sigma).

Immunogen: Fab fragment of human IgG

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% thimerosal

Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).