This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract. In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 µg of heat shocked HeLa cell lysate by western blotting.