Synonyms: Anti-TNRC6A; Anti-Trinucleotide repeat containing 6A
Storage: -20C
UNSPSC Code: 12352203
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Application: Anti-GW182 antibody produced in rabbit is suitable for use as a primary antibody for immunostaining of HeLa cells and human hepatoma cells. It is also suitable for indirect immunofluorescence at a working concentration of 2.5-5.0μg/mL using human epithelial HEp-2 cells.
Biochem/physiol Actions: GW182 is a phosphorylated cytoplasmic autoantigen. It contains multiple glycine-tryptophan (GW) repeats along with a single RNA recognition motif. GW bodies or P-bodies are cytoplasmic bodies that are involved in mRNA degradation, storage and translational repression. These bodies contain Argonaute proteins and m-RNAs that associate with GW182. GW182 is involved in post-transcriptional gene silencing/RNA interference (RNAi) via the microRNA pathway. Inhibition of gene expression leads to destruction of GWBs and disruption of RNAi and microRNA-induced gene silencing as it forms an integral component of GWBs.
Immunogen: synthetic peptide corresponding to amino acid residues 864-878 of mouse GW182, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.
Physical form: Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport