Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.
Synonyms: Anti-Gasdermin domain-containing protein 1; Anti-Gasdermin-D
Storage: -20C
Application: The antibody is recommended to use in various immunological techniques, including Immunoblot (>=53 kDa) and Immunofluorescence.
Biochem Physiol Actions: Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.
Other Notes: This product is for R&D use only, not for drug, household, or other uses.