Synonyms: Anti-Glucose Regulated Protein 75; Anti-Mitochondrial Hsp70; Anti-Mortalin; Anti-Peptide Binding Protein 74
MDL No.: MFCD00164609
Storage: -20C
UNSPSC Code: 12352203
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General description: GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing. GRP75/mortalin has been localized mainly to the endoplasmic reticulum (ER), but also to various cellular compartments including mitochondria and cytoplasmic vesicles.
Application: Anti-GRP75 antibody produced in rabbit is suitable for indirect immunofluorescence at a working concentration of 1-2mug/mL using HeLa cells and immunoblotting at 0.2-0.4mug/mL using whole cell extracts of the human epitheloid carcinoma HeLa cells, Madin-Darby canine kidney (MDCK) cells, and mouse fibroblasts NIH3T3 cells.
Biochem/physiol Actions: GRP75 (glucose-regulated protein 75) belongs to a subfamily of the heat shock proteins Hsp70. It is involved in stress responses to intracellular trafficking, antigen processing, and control of cell proliferation, differentiation, and tumorigenesis. It is induced by low levels of ionizing radiation, glucose deprivation, calcium ionophore, and ozone and its levels correlate with muscle activity, mitochondrial activity, and biogenesis.
Immunogen: synthetic peptide corresponding to amino acids 665-679 located at the C-terminus of human GRP75. This sequence is identical in mouse GRP75 (mot-1 and mot-2 isoforms) and highly conserved in rat (1 amino acid substitution).
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Target description: GRP75 (SQ-15) encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation.
RIDADR: NONH for all modes of transport
WGK Germany: 3