Guanine nucleotide-binding protein G(t) subunit alpha-1 (UniProt P11488; also known as Transducin alpha-1 chain) is encoded by the GNAT1 (also known as GNATR, CSNB1G, CSNBAD3) gene (Gene ID 2779) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha (alpha), beta (beta), and gamma (gamma) subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyze the GDP-to-GTP exchange of the Galpha subunit, causing a dissociation of the Gbetagamma dimer from the now GTP-bound Galpha subunit. The Galpha subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with Gbetagamma and re-starts the cycle. There exist at least 20 different Galpha subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Eight different genes code for Gi alpha subunits, including GNAT1 and GNAT2 that code for Gt in the rod (rTalpha by GNAT1) and cone (cTalpha by GNAT2) cells of the retina, where they function as the alpha subunit of the trimeric G protein transducin. The alpha-subunit binds GTP and activates cyclic GMP phosphodiesterase (PDE), whereas the beta and gamma subunits mediate interaction with rhodopsin. Mutations in RHO, PDE6B, and GNAT1 genes are associated with autosomal dominant congenital stationary night blindness (CSNB), and patients with mutations in these genes exhibit the Riggs- or Schubert-Bornschein-type electronegative electroretinogram (ERG).
Synonyms: Guanine nucleotide-binding protein G(t) subunit alpha-1, Transducin alpha-1 chain
Application: Anti-GNAT1 Antibody, clone 11A8.1 is an antibody against GNAT1 for use in Western Blotting.
Other Notes: Concentration: Please refer to lot specific datasheet.