?-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. It is related to a- and ß-tubulin and is a member of the tubulin superfamily of proteins. ?-Tubulin abundance is less than 1% of the level of either a- or ß-tubulin. ?-Tubulin shares approximately 28-32% identity with a-tubulin from various organisms and 32-36% identity with ß-tubulins. Some regions are highly conserved among a-, ß- and ?-tubulins.
Synonyms: Anti-gamma-Tubulin
Storage: -20C
Application: Anti-?-Tubulin antibody produced in rabbit has been used in
•immunofluorescence analysis
•western blotting
•immunohistochemistry
Biochem Physiol Actions: ?-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. ?-Tubulin is not a member of microtubule like a- and ß-tubulin. It functions as the microtubule nucleator at the microtubule organizing centre (MTOC). It binds to the ß-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the a-tubulin half exposed at the plus end.