This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Reactivity in other immunoassays is unknown. This product was assayed by western blotting after immunoprecipitation of mouse GADD45 gamma from a lysate containing over expressed protein. A 1:100 to 1:500 dilution shows reactivity with a band at 19.2kDa corresponding to mouse GADD45 gamma. Use 10 µL for immunoprecipitation.