MDL No.: MFCD00239679
Storage: -20C
UNSPSC Code: 12352203
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General description: G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.
Application: Anti-Galpha i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4muL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.
Biochem/physiol Actions: Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca+2 mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.
Immunogen: synthetic peptide corresponding to the C-terminal region of Galphai3.
Physical form: Solution of whole antiserum diluted in PBS, pH 7.4.
Specificity: Recognizes Galphai3 and Galpha0 G-proteins.
RIDADR: NONH for all modes of transport
WGK Germany: 3