The Ubiquitin-like protein FAT10, also known as Ubiquitin D (UBD) or Human Leukocyte Antigen (HLA)-F adjacent transcript 10 (FAT10), is a member of the Ubiquitin-like proteins (UBLs) modifier proteins.There are more than 12 identified UBLs including FAT10, SUMOs, NEDD8, ISG15, UFM1, URM1, ATG12, ATG8, FUB1 and HUB1.2 UBLs share a similar structure and function and all UBLs can be covalently attached to their target proteins via the E1-E2-E3 enzymatic cascade in a similar manner to Ubiquitin. UBLylation is a major post-translational modification that in many cases leads to protein degradation in the 26S proteasome.2
Synonyms: GABBR1; UBD; Ubiquitin D
Storage: -20C
Application: The antibody may be used in various immunochemical techniques including Immunoblotting and Immunoprecipitation. Detection of the FAT10 band by Immunoblotting is specifically inhibited by the immunogen.
Biochem Physiol Actions: FAT10 is a unique UBL containing a C-terminal GG motif available for activation and conjugation. This motif differentiates FAT10 from ubiquitin and other UBLs the needs to be cleaved from precursors. Furthermore, unlike ubiquitin that requires a poly-ubiquitin chain, the FAT10 can bind the 26S proteasome as a monomer to mediate proteasomal degradation.2-3 Under normal conditions, FAT10 have short half-life, its expression is repressed by p534 and is restricted to tissues of the immune system like thymus, lymph nodes and the spleen. Inflammatory cytokines such as IFN? and TNFa and downstream STAT3 and NF-?B signaling can induce its expression also in other tissues.2-3,5 FAT10 expression is also upregulated and can be increased up to 100-fold in many cancer types including liver and colon cancer HCC, colorectal, ovarian and uterine carcinomas triple-negative breast cancer, bladder cancer, gastric cancer, glioma, pancreatic ductal, adenocarcinoma and osteosarcoma.2,5-6 FATylation has an important role in many cellular process varied from cell maintenance to DNA repair. Understanding FAT10 mechanism and finding its downstream substrates is important to reveal its role in tumorigenesis.1-7