This Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 is validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), ELISA and Flow Cytometry for the detection of DNA G-quadruplex.
Alternate Names: DNA G-quadruplex (G4)
Format: Purified
Presentation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host: Mouse
Specificity: Clone 1H6 binds both tetramolecular and unimolecular (intramolecular) DNA G-quadruplex (G4) structures without sequence specificity, while exhibiting significantly lower affinity toward tetramolecular RNA structure or a triplex DNA structure, and little to no affinity toward either non-G4 ssDNA or ddDNA. However, clone 1H6 does not seem to bind the [AGGG(TTAGGG)3] unimolecular G4 structure, indicating a broad selectivity toward many, but not all, DNA G4 structures (Henderson, A., et al. (2014). Nucleic Acids Res. 42(2):860-869).
Isotype: IgG2bκ
Species Reactivity: All
Antibody Type: Monoclonal Antibody
Purification Method: Protein G Purified
Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.
Key Applications
- Immunocytochemistry
- Immunohistochemistry (Paraffin)
- ELISA
- Flow Cytometry