Storage: 2-8C
UNSPSC Code: 12352200
Features and Benefits: . Specific to free and bound digoxigenin. The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
General description: Monoclonal antibody to digoxigenin.Balb/c mice were immunized with edestin-bound digoxigenin. Spleen cells were subsequently fused with myeloma cells of cell line P3X63Ag8.653. Antibodies were purified by gel filtration, diluted in 10 mM potassium phosphate buffer, 75 mM NaCl, 5% raffinose (w/v), 0.01% 2-methylisothiazolone (v/v), pH 7.8, and lyophilized.
General description: Contents: Lyophilizate
Application: Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using: . ELISA. Immunohistocytochemistry. In situ hybridization. Western blot. Immunofluorescence staining. FISH (fluorescent in situ hybridization)
Reconstitution: Add 1 ml double-distilled water to a final concentration of 100 mug/ml.
Specificity: The monoclonal antibody reacts with free and bound digoxigenin.
Analysis Note: There are no cross reactivities known.
Preparation Note: Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: for coating: 2 to 4 μg/ml• Immunohistocytochemistry: 0.5 to 2 μg/ml• In situ hybridization: 0.2 to 0.4 μg/ml• Western blot: 0.5 to 2 μg/mlWorking solution: For coating applicationsphosphate buffered saline, pH 7.4
Other Notes: For life science research only. Not for use in diagnostic procedures.
RIDADR: NONH for all modes of transport