Dendrin (UniProt: O94850) is encoded by the DDN (also known as KIAA0749) gene (Gene ID: 23109) in human. Dendrin is a proline-rich cytoplasmic protein that was originally recognized in telencephalic dendrites of sleep-deprived rats. Dendrin is specifically expressed in the brain and in kidneys. In the brain, it interacts with alpha-actinin and possibly modulates the structure of the actin cytoskeleton. In kidney it plays a role in apoptosis of kidney glomerular podocytes. Dendrin contains two putative nuclear localization signals (NLSs) and three PPXY motifs that are conserved among human, rat, and mouse. Dendrin is reported to interact with alpha-actinin and Maguk with inverted orientation (MAGI) or synaptic scaffolding molecule (S-SCAM). Interaction with S-SCAM is shown to be responsible for the retention of dendrin in the cytoplasm of mammalian cells and prevent its nuclear import. In experimental glomerulonephritis, dendrin is shown to relocate from the slit diaphragms (SD) to the nucleus of injured podocytes. High-doses of TGF- 1 can directly promote the import of dendrin into the nucleus and in the nucleus Dendrin enhances staurosporine- and TGF- 1-mediated apoptosis. Acute nicotine treatment is reported to induce a rapid, but short-lived, expression of the dendritically targeted, corticolimbic mRNA dendrin in specific regions of adolescent brain. (Ref.: Schochet, TL et al (2008). Eur.J. Neurosci. 28(10):1967-79; Kremerskothen, J et al. (2006). J. Neurochem. 96(6):1659-66; Asanuma, K et al. (2007). Proc. Natl. Acad. Sci. USA. 104(24): 10134-39).
Synonyms: DDN
Application: Western Blotting Analysis: 2 µg/mL from a representative lot detected Dendrin in 10 µg of human hippocampus tissue lysate.Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Dendrin in rat cerebral cortex, mouse cerebellum, and rat kidney tissue.
Other Notes: Concentration: Please refer to lot specific datasheet.