This antibody reacts with human Cul5 by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using 293T, U2OS and others). This antibody also co-immunoprecipitates associated proteins. A 90.9 kDa band corresponding to human Cul5 is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications.