This antibody reacts with human Cul3 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). An 88.9 kDa band corresponding to human Cul3 is detected. Most cell lines expressing Cul3 can be used as a positive control. Researchers should determine optimal titers for other applications.