This antibody reacts with human Cul1 by immunohistochemistry, western blot, and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, but rather use 0.1% SDS. An 89.6 kDa band corresponding to human Cul1 is detected. Most cell lines expressing Cul1 can be used as a positive control. Researchers should determine optimal titers for other applications.