Storage: -10 to -25C
Application: Quality Control TestingEvaluated by Flow Cytometry in HT1080 cells transfected with human Claudin-2.Flow Cytometry Analysis (FC): 1 μg of this antibody detected Claudin-2 in one million HT1080 cells transfected with human Claudin-2.Tested ApplicationsFlow Cytometry Analysis: A representative lot detected Claudin-2 in Flow Cytometry applications (Takigawa, M., et. al. (2017). J Pharmacol Exp Ther. 363(3):444-451).Inhibition Assay: A representative lot of this antibody inhibited TNFa-induced dysfunction of tight junction seals in the intestinal epithelium. (Takigawa, M., et. al. (2017). J Pharmacol Exp Ther. 363(3):444-451). Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.