Cell division control protein 42 homolog (UniProt: P60953; also known as G25K GTP-binding protein) is encoded by the CDC42 gene (Gene ID 998) in human. CDC42 homolog is a plasma membrane-associated small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state. Its activity is regulated by guanine nucleotide exchange factors (GEFs), which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) that increase the GTP hydrolysis activity, and GDP dissociation inhibitors, which inhibit the dissociation of the nucleotide from the GTPase. In the active state it binds to a variety of effector proteins to regulate cellular responses. Cdc42 is known to regulate the dynamic organization of the cytoskeleton and membrane trafficking for physiologic processes such as cell proliferation, motility, polarity, cytokinesis and growth. Deregulation of Cdc42 is found in several pathogenic processes such as cancer, cardiovascular diseases, and neuronal degenerative diseases. It is involved in epithelial cell polarization processes and regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. During the prometaphase stage, it localizes to spindle and moves to the central spindle as cells progress through anaphase to telophase.
Synonyms: Cell division control protein 42 homolog, G25K GTP-binding protein
Application: Western Blotting Analysis: A representative lot detected GFP-tagged recombinant CDC42 brain isoform (Cdc42-palm), but not the placenta isoform Cdc42-prenyl exogenously expressed in COS-7 cells (Courtesy provided by Rujun).Immunocytochemistry Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) at the axon tip by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized DIV3cortical neurons from Zdhhc8-deficient mice (Mukai, J., et al. (2015). Neuron. 86(3):680-695).Western Blotting Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) in whole cell lysate and among purified palmitoylated proteins (PPs) from cultured rat cortical embryonic neurons (Kang, R., et al. (2008). Nature. 456(7224):904-909).Western Blotting Analysis: A representative lot detected CDC42 brain isoform (Cdc42-palm) in rat brain membrane fractions (Kang, R., et al. (2008). Nature. 456(7224):904-909).
Other Notes: Concentration: Please refer to lot specific datasheet.