Anti-CD81 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry, and Immunofluorescence. Expect a band at approximately 26 kDa in Western Blots using positive control Human lung tissue, which can be used for IHC, A549 whole cell lysates for IF, or other specific cell lysates and tissues. Specific conditions for reactivity should be optimized by the end user.