Caspases are a family of intracellular proteases that mediate cell death and the principal effectors of apoptosis. There are 14 different caspases have identified which can be grouped into three different subfamilies based on their substrate specificities. Caspase 3 is also known as CPP32, Yama, apopain. Among all the types, caspase 3, a cytosolic protein, found in cells as an inactive 32 kDa proenzyme.
Synonyms: Activated Caspase 3 Antibody; Activated Caspase 3 Antibody - Anti-Caspase 3, Active antibody produced in rabbit; Anti-Apopain; Anti-CPP32; Anti-Yama
MDL Number: MFCD01321906
Storage: -20C
Application: Anti-Caspase 3, Active antibody has been used:
•for western blotting of cytochrome c for caspase activation
•as primary antibody in immunofluorescence staining of embryos and postnatal mice cryosections
•in Western blot analysis of activated caspase 3
•as a primary antibody in immunodetection of rat brain sections
Biochem Physiol Actions: Caspase 3 is one of the key effectors of apoptosis. It acts downstream of caspase 9 in the apoptotic pathway. During apoptosis, it gets activated by proteolytic cleavage into the 17-19 kDa (p17, p18) and 12 kDa (p12) active subunits. Any alteraion in CASP-3 in mice, causes premature lethality. It has also shown that the deletion of gene affects brain development resulting of hyperplasias and disorganized cell deployement. Clinically it is related to Huntington disease (HD). Caspase 3 brings about cell death by activating CAD (caspase activated DNAse I), an endonuclease that causes degradation of chromosomal DNA.