This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2.This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot.