Anti-beta-Tubulin III antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

Synonyms: Anti-Tuj 1

MDL No.: MFCD01323870

Storage: -20C

UNSPSC Code: 12352203

General description: Tubulin β 3 class III (TUBB3) also known as β-Tubulin III, is encoded by the gene mapped to human chromosome 16q24.3. TUBB3 protein expression is restricted to neurons.α/β-Tubulin, an integral component of microtubules, is present in almost all eukaryotic cells.Anti-β-Tubulin III is developed in rabbit using as immunogen a synthetic peptide corresponding to amino acid residues 441-450 of human β-tubulin III (Ala 446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals. α/β-Tubulin occurs mostly as soluble (approx. 100-110 kDa) heterodimeric sets of α- and β-tubulin isotypes or as polymers in assembled microtubules. β-Tubulin III (also designated β-4 chain) is found in the brain and dorsal root ganglia and appears to be localized to neurons of the central and peripheral nervous system. β-tubulin III is also found in Sertoli cells of the testis, spermatozoa tails, certain lung cells, and apparently some breast stromal cells.

Application: Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:. Immunofluorescence.. Immunostaining.. Western blotting.

Application: Anti-β-Tubulin III antibody produced in rabbit has been used in following studies:. Immunofluorescence. Immunostaining. Western blotting. Indirect immunoperoxidase assay (IPA)

Biochem/physiol Actions: Tubulin β 3 class III (TUBB3) plays a vital role in nervous system development and axon guidance. Mutation of the gene encoding protein leads to the ocular motility disorder, congenital fibrosis of the extraocular muscle type 3 (CFEOM3), and various neurological syndromes. Altered expression of the protein affects microtubule dynamics and microtubule-kinesin interactions. Increased expression of TUBB3 is observed in tumor tissues. Decreased expression of TUBB3 can be considered as an important marker for poor prognosis of cutaneous malignant melanoma. TUBB3 is implicated in the suppression of invasive growth of tumor tissue;therefore, it is considered to be a potential target for development of antitumor drugs.TUBB4 is responsible for microtubule mediated N-cadherin transport to the cell membrane, which is necessary for cell-cell contact formation. TUBB4 participates in the progression of osteosarcoma. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, cell morphogenesis, and other cytoskeletal functions. Increased β-Tubulin III expression is observed during axonal growth. β-tubulin III is also found in certain tumors of non-neural origin, such as lymphoma, squamous cell carcinoma, giant cell glioblastoma multiforme, oligodendroglioma, and malignant melanoma but not in their non-neoplastic adult counterparts. Its expression is increased in various chemoresistant epithelial cell lines and gliomas and lung cancer with ascending grade of malignancy.

Immunogen: synthetic peptide corresponding to amino acid residues 441-450 of human beta-tubulin III (Ala446 to Ser446 substitution) with N-terminal added cysteine, conjugated to KLH. The sequence is conserved in mammals.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity: Anti-beta-Tubulin III recognizes human, mouse,and rat beta-tubulin III.

Preparation Note: Storage and Stability: For continuous use, store at 2-8 C for up to one month.For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Other Notes: In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working concentrations by titration.