Synonyms: Anti-GUS
MDL No.: MFCD00282142
Storage: -20C
UNSPSC Code: 12352203
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General description: Reporter genes are widely used for studying the expression of foreign genes in transformed plants tissues. Using appropriate promoter-reporter gene constructs, this technique allows an independent verification of the transformed status of tissues growing on media containing selective antibiotics or herbicides. In addition, it serves as a principal means to follow gene transfer and monitor genetic transformation of plant species. Several screenable markers are available including beta-glucuronidase (GUS), beta-galactosidase (beta-GAL), chloramphenicol acetyl transferase (CAT), green fluorescent protein (GFP) and luciferase. E. coli GUS has been extensively used to monitor transgene delivery to plant tissue. Encoded by the E. coli gus gene (also referred to as uidA), GUS protein (60 kDa) is an hydrolase that catalyses the cleavage of a variety of b-glucuronide derivatives available for colorimetric, fluorimetric and histochemical assays. Several features make the gus gene superior as a reporter gene for plant studies and in the production of genetically engineered crops. Many plants lack detectable endogenous glucuronidase activity, resulting in essentially no background. In addition, GUS activity is easily assayed in vitro and can withstand fixation, enabling histochemical localization in cells and tissue sections. However, one of the major limitations of the gus reporter gene system is that histochemical GUS assay system is destructive for the plant tissue, and therefore it is not suitable for direct visual selection of transformed plants. Antibodies specific for GUS are useful tools for detecting GUS gene product in transformed plants.
General description: Rabbit polyclonal anti-beta-Glucuronidase (N-Terminal) antibody recognizes bacterial GUS expressed in transgenic tobacco plants. Staining of the GUS band in immunoblotting is specifically inhibited by the immunizing GUS peptide (E. coli, amino acids 1-16).
Application: Rabbit polyclonal anti-beta-Glucuronidase (N-Terminal) antibody may be used for the detection of GUS by immunoblotting (60 kDa). It may be used to detect the reporter GUS marker protein in transgenic plants.
Immunogen: sythetic peptide corresponding to amino acids 1-16 located at the N-terminus of E. coli GUS, conjugated to KLH.
Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
RIDADR: NONH for all modes of transport
WGK Germany: 3