Anti-ASC Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry-P, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 22 kDa in Western Blots of specific cell lysates and tissues. Positive controls used are HL60 cells, human spleen tissues, and THP-1 cells.